Improved eukaryotic promoter-detection vector carrying two luciferase reporter genes
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چکیده
منابع مشابه
Improved eukaryotic promoter-detection vector carrying two luciferase reporter genes.
BENCHMARKS Understanding gene regulation is of fundamental importance to virtually all biological systems. A gene is regulated through cis-acting regulatory DNA sequences (elements) in conjunction with transacting regulatory proteins. The cis-acting regulatory elements involved in transcription include, for example, the promoter, enhancer, silencer, specific activator binding sites, and specifi...
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Luciferase is used as a reporter enzyme to estimate gene expression in prokaryotic or eukaryotic cells because the amount of luciferase present is correlated with light intensity in the presence of excess luciferin (1). Furthermore, its sensitivity and range of linear responses are superior to those of other typical reporters (2). When the luciferase gene containing a target promoter region is ...
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متن کاملApplication of the dual-luciferase reporter assay to the analysis of promoter activity in Zebrafish embryos
BACKGROUND The dual-luciferase assay has been widely used in cell lines to determine rapidly but accurately the activity of a given promoter. Although this strategy has proved very useful, it does not allow the promoter and gene function to be analyzed in the context of the whole organism. RESULTS Here, we present a rapid and sensitive assay based on the classical dual-luciferase reporter tec...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2003
ISSN: 0736-6205,1940-9818
DOI: 10.2144/03356bm05